Badania
Lencomm Trade

Odczynniki, reagenty oraz aparatura do badań naukowych

Nowe produkty do badań naukowych

ChIP kits

Enzyme-based and sonication based ChIP kits

Dzięki współpracy CST i NEB’a powstał wysokiej jakości odczynnik chemiczny (SimpleChIPTM Enzymatic Chromatin IP Kit). Kit ten może być z powodzeniem stosowany w celu wykrywania endogennego poziomu interakcji białko-DNA oraz modyfikacji histonów w komórkach ssaczych. (a) #9002 SimpleChIPTM Enzymatic Chromatin IP Kit (Agarose Beads) (b) #9003 SimpleChIPTM Enzymatic Chromatin IP Kit (Magnetic Beads)
Wskazano na wyższość enzymatycznego trawienia chromatyny, w porównaniu z sonikacją, jako że:
– trawienie enzymatyczne jest łagodniejsze niż sonikacja, a co za tym idzie lepiej zachowuje integralność chromatyny i epitopów dla przeciwciał, co w następstwie znacznie zwiększa efektywność immunoprecypitacji;

– wzrost wydajności immunoprecypitacji białek docelowych przekłada się na wzrost detekcji wiązań typu białko-DNA;

– wzrost wykrywalności docelowych loci DNA przekłada się na mniejsze ilości chromatyny, która musi zostać zużyta do badań, a co za tym idzie zmniejsza się zużycie odczynników i skróceniu ulega czas niezbędny do przeprowadzenia eksperymentu;

– trawienie enzymatyczne pozwala na zachowanie większej specyficzności substratowej (z zachowaniem mniejszego tła), co jest istotne w badaniach DNA wiążącego się z czynnikami transkrypcyjnymi i kofaktorami.

CST-Newly Released Products List - August 2010

SKU Product Name Size Species x-Reactivity Applications Product Category Type
4083S
DAPI
1 mg
All
IF-F IF-IC IF-P
Related Products
Cellular Dyes
4530S
Notch2 (D67C8) XP(tm) Rabbit mAb
100 ul
H R
W IP IF-IC
Developmental Biology
Monoclonal Antibody
4666S
WRN (8H3) Mouse mAb
100 ul
H M
W
Cell Cycle / Checkpoint Control
Monoclonal Antibody
5018S
Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP(tm) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
100 ul
IF-IC F
IF-IC F
Translational Control
Monoclonal Antibody
5059S
alpha-Tubulin (11H10) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
100 ul
H M R Mk Dm B (Dg)
IF-IC
Cytoskeletal Signaling
Monoclonal Antibody
5078P
Phospho-4E-BP1 (Thr70) Antibody
40 ul
H M R Mk
W IP
Translational Control
Polyclonal Antibody
5078S
Phospho-4E-BP1 (Thr70) Antibody
100 ul
H M R Mk
W IP
Translational Control
Polyclonal Antibody
5108S
EGF Receptor (D38B1) XP(tm) Rabbit mAb (Alexa Fluor(R) 555 Conjugate)
100 ul
H
IF-IC
Tyrosine Kinase
Monoclonal Antibody
5154LF
Human Latent Transforming Growth Factor beta-1 (hLatent TGF-beta-1)
10 ug
 
 
Immunology and Inflammation
Growth Factors and Cytokines
5154LC
Human Latent Transforming Growth Factor beta-1 (hLatent TGF-beta-1)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5173P
Phospho-CD79A (Tyr182) Antibody
40 ul
H (M) (R)
W IP IF-IC F
Immunology and Inflammation
Polyclonal Antibody
5173S
Phospho-CD79A (Tyr182) Antibody
100 ul
H (M) (R)
W IP IF-IC F
Immunology and Inflammation
Polyclonal Antibody
5204SF
Mouse Interleukin-1-beta (mIL-1-beta)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5204SC
Mouse Interleukin-1-beta (mIL-1-beta)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5204LF
Mouse Interleukin-1-beta (mIL-1-beta)
50 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5204LC
Mouse Interleukin-1-beta (mIL-1-beta)
50 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5231LF
Mouse Transforming Growth Factor beta-1 (mTGF-beta-1)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5231LC
Mouse Transforming Growth Factor beta-1 (mTGF-beta-1)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5234LC
Human FGF acidic (hFGF acidic)
50 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5234SC
Human FGF acidic (hFGF acidic)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5234SF
Human FGF acidic (hFGF acidic)
10 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5234LF
Human FGF acidic (hFGF acidic)
50 ug
   
Immunology and Inflammation
Growth Factors and Cytokines
5258S
Fetuin A (F180) Antibody
100 ul
H
W IP
Developmental Biology
Polyclonal Antibody
5266S
Frizzled5 (D2H2) Rabbit mAb
100 ul
H
W IP
Developmental Biology
Monoclonal Antibody
5311S
PTP1B Antibody
100 ul
H
W
Phosphatases
Polyclonal Antibody
5321S
MDA-5 (D74E4) Rabbit mAb
100 ul
H M
W IP
Immunology and Inflammation
Monoclonal Antibody
5326P
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP(tm) Rabbit mAb
40 ul
H M R Mk
W IF-IC ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5326S
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP(tm) Rabbit mAb
100 ul
H M R Mk
W IF-IC ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5327P
Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb
40 ul
H M R Mk
W IP IF-IC ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5327S
Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb
100 ul
H M R Mk
W IP IF-IC ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5347S
LAT1 Antibody
100 ul
H
W IP
Glucose / Energy Metabolism
Polyclonal Antibody
5351S
hnRNP Q/R Antibody
100 ul
H
W IP
Translational Control
Polyclonal Antibody
5361S
JARID1C (D29B9) Rabbit mAb
100 ul
H M (Mk) (Pg) (Hr)
W IP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5398S
KHSRP Antibody
100 ul
H M R Mk
W
Translational Control
Polyclonal Antibody
5410P
Acetyl-Histone H2B (Lys12) Antibody
40 ul
H M R Mk
W IP
Chromatin Regulation / Nuclear Function
Polyclonal Antibody
5410S
Acetyl-Histone H2B (Lys12) Antibody
100 ul
H M R Mk
W IP
Chromatin Regulation / Nuclear Function
Polyclonal Antibody
5416S
DDB2 (D4C4) Rabbit mAb
100 ul
H M (R) (Mk)
W
Protein Folding and Stability
Monoclonal Antibody
5421S
Bub1b (D32E8) Rabbit mAb
100 ul
H
W
Cell Cycle / Checkpoint Control
Monoclonal Antibody
5427P
Di-Methyl-Histone H3 (Lys79) (D15E8) XP(tm) Rabbit mAb
40 ul
H M R Mk
W ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5427S
Di-Methyl-Histone H3 (Lys79) (D15E8) XP(tm) Rabbit mAb
100 ul
H M R Mk
W ChIP
Chromatin Regulation / Nuclear Function
Monoclonal Antibody
5440S
LRP5 (D5G4) Rabbit mAb
100 ul
H M R Mk
W
Developmental Biology
Monoclonal Antibody
442S
RBPSUH Antibody
100 ul
 M R Mk
W
Developmental Biology
Polyclonal Antibody
5443S
Ikaros Antibody
100 ul
H M R
W IP IF-IC
Immunology and Inflammation
Polyclonal Antibody
5447S
EpCAM (VU1D9) Mouse mAb (Alexa Fluor(R) 647 Conjugate)
100 ul
H
IF-IC F
Cytoskeletal Signaling
Monoclonal Antibody
5461S
Synaptophysin (D35E4) XP(tm) Rabbit mAb
100 ul
H M R
W IP IF-F
Neuroscience
Monoclonal Antibody
5467S
Synaptophysin (D40C4) Rabbit mAb
100 ul
H M R
W IP
Neuroscience
Monoclonal Antibody
5482P
Phospho-NDRG1 (Thr346) (D98G11) XP(tm) Rabbit mAb
40 ul
H M R Mk
W IP IHC-P IF-IC F
Akt Signaling Pathways
Monoclonal Antibody
5482S
Phospho-NDRG1 (Thr346) (D98G11) XP(tm) Rabbit mAb
100 ul
H M R Mk
W IP IHC-P IF-IC F
Akt Signaling Pathways
Monoclonal Antibody
5483P
Phospho-TBK1/NAK (Ser172) (D52C2) XP(tm) Rabbit mAb
40 ul
H (M) (R) (Mk) (X) (B) (Dg)
W IP IF-IC F
Immunology and Inflammation
Monoclonal Antibody
5483S
Phospho-TBK1/NAK (Ser172) (D52C2) XP(tm) Rabbit mAb
100 ul
H (M) (R) (Mk) (X) (B) (Dg)
W IP IF-IC F
Immunology and Inflammation
Monoclonal Antibody
5513S
Estrogen Receptor beta Antibody
100 ul
H M R Mk
W
Nuclear Receptor Signaling
Polyclonal Antibody
5580S
NMDAR2B (N59/36.1) Mouse mAb
100 ul
M R
W IP
Neuroscience
Monoclonal Antibody
5673S
TIMP3 (D74B10) Rabbit mAb
100 ul
H M R Mk
W
Protein Folding and Stability
Monoclonal Antibody
5682S
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP(tm) Rabbit mAb (PE Conjugate)
100 ul
H M R Mk Mi Dm Z B Dg Pg Sc (Ce)
F
MAP Kinase Signaling
Monoclonal Antibody
5736S
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose Bead Conjugate)
400 ul
H M R Hm Mk Mi Z B Dg Pg Sc Ce
IP
MAP Kinase Signaling
Monoclonal Antibody
5738S
TIMP2 (D18B7) Rabbit mAb
100 ul
H M Mk
W
Protein Folding and Stability
Monoclonal Antibody
5740S
Arf6 (D12G6) Rabbit mAb
100 ul
H M R Mk
W IP
Protein Folding and Stability
Monoclonal Antibody
6282S
SignalSilence(R) HER2/ErbB2 siRNA I
300 ul
H
 
Tyrosine Kinase
siRNA
6283S
SignalSilence(R) HER2/ErbB2 siRNA II
300 ul
H
 
Tyrosine Kinase
siRNA
6302S
SignalSilence(R) FoxO3a siRNA I
300 ul
H
 
Akt Signaling Pathways
siRNA
6303S
SignalSilence(R) FoxO3a siRNA II
300 ul
H
 
Akt Signaling Pathways
siRNA
6304S
SignalSilence(R) PARP siRNA I
300 ul
H
 
Apoptosis / Caspase Signaling
siRNA
6305S
SignalSilence(R) PARP siRNA II
300 ul
H
 
Apoptosis / Caspase Signaling
siRNA
6327S
SignalSilence(R) I-kappa-B-alpha siRNA I
300 ul
H
 
Immunology and Inflammation
siRNA
6596S
SignalSilence(R) Frizzled6 siRNA I
300 ul
H
 
Developmental Biology
siRNA
6597S
SignalSilence(R) Frizzled6 siRNA II
300 ul
H
 
Developmental Biology
siRNA
7038S
PathScan(R) Total p70 S6 Kinase Sandwich ELISA Kit
1 Kit
H M
ELISA
Screening Technologies
ELISA Kit
7255S
PhosphoPlus(R) Akt (Ser473) In-Cell Duet (ICW Compatible)
1 Kit
H M R Mk
ICW
Screening Technologies
Antibody Cocktail Kit
7257S
PhosphoPlus(R) p38 MAPK (Thr180/Tyr182) In-Cell Duet (ICW Compatible)
1 Kit
H M R Mk
ICW
Screening Technologies
Antibody Cocktail Kit
7261S
PhosphoPlus(R) S6 Ribosomal Protein (Ser235/236) In-Cell Duet (ICW Compatible)
1 Kit
H M R Mk
ICW
Screening Technologies
Antibody Cocktail Kit
7263S
PhosphoPlus(R) p44/42 MAPK (Erk1/2)(Thr202/Tyr204) In-Cell Duet (ICW Compatible)
1 Kit
H M R Mk
ICW
Screening Technologies
Antibody Cocktail Kit
7761S
PathScan(R) Phospho-FLT3 (panTyr) Sandwich ELISA Kit
1 Kit
H
ELISA
Screening Technologies
ELISA Kit